The ONEcleaner Surgical Instrument Detergent 4 Enzyme Cleaners are non irritating, biodegradable, neutral pH, phosphate free, and deliver the 4 enzyme detergents that are necessary to effectively breakdown and remove bioburden from surgical instruments. Surgical instrument detergent enzyme cleaners that do not contain these four enzyme detergents cannot remove all forms of proteinaceous bioburden. For the surgical instrument detergent cleaning process to be thorough, four surgical instrument cleaner enzyme detergents are needed. Lipase enzyme detergent cleaners breakdown fat to cleave fatty acid residue from the glycerol residue in a neutral fat or a phospholipid. Amylase enzyme detergent cleaners breakdown starch to catalyze the hydrolysis of starch to sugar to produce carbohydrate derivatives. Carbohydrate enzyme detergent cleaners breakdown starch to a lower level to catalyze the hydrolysis of higher carbohydrates to lower forms. Protease enzyme detergent cleaners breakdown blood including the proteinases and peptidases, to catalyze the hydrolytic breakdown of proteins. The optimal temperature for maximum enzymatic enzyme detergent cleaning performance peaks at 137 degrees Fahrenheit 58.33 degree Celsius. The cleaning activity of the enzyme detergents at temperatures below and above this point is less but does offer cleaning value. The cleaning activity of the enzyme detergents does not stop at this temperature but is does lessen as the temperature increases. Enzyme Surgical Instrument Cleaners and four enzyme endoscope cleaners boost cleaning power. Lubricating with surgical instrument cleaners while cleaning speeds the cleaning process and renders the highest quality cleaning outcomes. ONEcleaner four enzyme surgical instrument and endoscope cleaners deliver lower cleaning costs. One application will remove encrustation and stains. ONE gallon delivers 512 gallons of highly concentrated enzyme surgical instrument and endoscope cleaners.